Fig. 2. Obesity promotes lost in the energy homeostasis and prone to permeability transition pore opening. (A) Mechanical performance index and energetic metabolites were evaluated in presence and absence of isoproterenol (ISO; data from 3 independent experiments) (B) Ca2+ retention capacity (CRC) of mitochondria in presence or absence of ADP and its representative trace of simultaneous measurements of crescent concentrations of Ca2+ with or without CsA. The arrow indicates the addition of 10 nM Ca2+. (C) Half-time Ca2+ release in the presence of CAT and ADP and a representative trace (data from 6 independent animals). Isolated mitochondria from hearts were analyzed for (D) ANT and (E) CypD expression by western blot; VDAC expression was used as a reference (data from 5 independent experiments). The error bars indicate the SEM. A t-test was performed to obtain the statistical analysis. Green bars indicate CTL groups and red bars 12-mo OB groups. In A: *p<0.05 vs no ISO; In A-E: **p<0.01 vs CTL.